Zhang Lin took out the lifting tube from the liquid nitrogen tank, then took out a freezing storage tube from the lifting tube, and then carefully put the lifting tube back because there were still many freezing tubes for other cells in the lifting tube. After Zhang Lin sealed the lock cover of the liquid nitrogen tank, he began a series of patient and meticulous operations.

He first put the frozen storage tube in a water bath pot, thawed it quickly with 37℃ hot water, then changed the centrifuge tube and centrifuged it with a centrifuge for five minutes, then sat down next to the ultra-clean workbench, baked the mouth of the centrifuge tube to the alcohol lamp, disinfected it, discarded the waste liquid, added fresh culture medium with a pipette, and repeatedly sprayed and mixed...

Gu Jun kept watching carefully from the side, and asked Senior Brother Zhang for advice if he had any questions and learned a lot.

Such as the speed control of the centrifuge, the suction and release control of the pipette, etc., these resuscitation operations will affect the survival rate and proliferation rate of cells.

Finally, Zhang Lin injected the cell suspension into the culture dish, added an appropriate amount of culture medium, and then put it into a cell incubator of 37℃ and 5% CO2.

"Wait, you will have to take care of it for at least two days." Zhang Lin took off his mask and pushed his glasses. "I suggest you take care of it for a week, so that the tumor rate can be guaranteed."

"If you keep for two days, try one batch first." Gu Jun shrugged and said, "There is no way, there is not enough time. The system shows that his condition will worsen in 30 days. If it is true, it may be difficult for him to get out of bed and walk. He said again: "Senior brother, go out for a meal tonight? I'll treat you."

"Forgive." Zhang Lin waved his hand, "I will go to feed the mice to the night, and I'll treat them."

...

In the next two days, Gu Jun spent all the time on campus. In addition to gathering with friends, he was reading books and reviewing and preparing for a make-up exam. If he couldn't die but had to stay in grades, it would be quite troublesome; the other was to go to the experimental building to take care of the cells and change the culture medium every day.

On the morning of the third day, Zhang Lin came to see it in person and said that the cells were barely good. He helped to carry out subculture and prepare cell suspension.

Gu Jun went to the school's experimental animal center. This was actually a large breeding base, located a few hundred meters away from the school campus. He was very lonely, maybe because it was filled with the stench of various animals.

He bought five nude mice, each of which was more than 100 yuan, which was quite expensive. Fortunately, he still had 20,000 yuan in savings, but he couldn't last long.

I regretted that I had little money when I was using it. Gu Jun really regretted that he donated the money so early... People are still alive, but the money is gone.

Cough cough. Each of these nude mice is only two or three fingers in size, and has no hair. They are packed in a sterile box. Once they come into contact with the outside bacterial environment, they will easily be infected and become sick and die. Therefore, the usual feeding and experimenting process needs to be carried out in a barrier environment.

The Barrier Environment Laboratory of Dongda University is located in the Experimental Animal Research Center, and this five-story yellow brick wall building stands next to the Experimental Building.

Gu Jun and Zhang Lin met in the lobby on the first floor of the research center and booked a one-hour use right for a small nude mouse room. This is a small room with only 21 square meters, divided into a front room and a back room. The front room has a laboratory bench and instruments, and the back room is a feeding room.

Before entering, they and all the items they brought had to undergo strict ultraviolet sterilization. The two of them set off in the nude mouse room with their special full-body protective clothing, masks, gloves, foot covers and other equipment.

Soon, at the front room laboratory bench, Gu Jun marked the five nude mice by punching, and then injected tumor cells subcutaneously into the right side of their hips.

Zhang Lin was responsible for assisting him. In theory, these cells could become tumors when they hit humans. Now, seeing the rich man Jun is so excited about the nude mice, he is sure that this little brother is not trying to murder.

After all the five nude mice were injected, Gu Jun put them in the feeding cage, and then it depends on whether they can become tumors.

"Senior brother, thank you." Gu Jun praised Senior Brother Zhang. It would definitely not be so smooth without Senior Brother's help.

"The old Chinese doctor you mentioned..." Zhang Lin still looked alert with Conan, "Isn't he really a liar?"

...

In the next week, Gu Jun continued his previous life rhythm, but he went to the nude mouse room every day to see the nude mice. Their feeding was taken care of by the managers of the nude mouse room, and each person had a daily meal and boarding fee of 3 yuan.

This week he only completed three ordinary tasks because those who need to be cured and saved cannot do it. Although the hospital is right next to him, he is not a doctor. The tasks he completed are related to medical exercises. There are already 5 boxes of pills on his hands, and Tairan's proficiency reaches the first level (3000/5000).

On the seventh day, Gu Jun walked into the nude mice room again in sterile equipment and observed the nude mice one by one.

None of them died. A round-like tumor as big as a soybean had grown under the right side of the buttocks of No. 1. The diameter was 0.5cm; No. 3 and No. 4 also had obvious protrusions, with diameters of 0.3cm and 0.5cm respectively; No. 2 and No. 5 still showed no signs of tumor formation.

At this point he could be sure that five nude mice and three nude mice became tumors.

Gu Jun set two nude mice to group A, one nude mice to group B, and two nude mice to group C and D were divided into two nude mice. They also bought 10 healthy nude mice at the animal center, 5 were set to group E and 5 were set to group F. Group A, group C and group E will receive the same dose of "variant medicine" (his name) every day; while Group B, group D and group F will not have any medication, and will only be used as a control purpose.

That is, nude mice that are tumor-abundant, nude mice that are not tumor-abundant and healthy nude mice have one group to take medication, and one group does not take medication.

At this time, six cages had been divided on the laboratory bench, and the nude mice were chirping and making a lot of fun.

Gu Jun was pounding 25 capsules of heterologous medicine capsules. Since the system stated that one box can extend the lifespan of 5 days, one box of 5 tablets should be one tablet a day. This is the dosage of adult humans, and the average weight of these nude mice is less than 20g. If they also have one tablet a day, they will probably be sacrificed immediately.

He poured out all the medicine powder in a capsule and weighed the dose, then converted the dose of the nude mice based on his weight, made it into a solution, and then diluted it in proportion.

Soon, Gu Jun held the gavage needle with the potion in one hand and grabbed a fixed healthy nude mouse with the other hand, and began the first gavage and medication.

"Jijiji." The nude mouse struggled a little, but he still pushed this potion into the potion and made its contribution to medicine.

At this moment, Gu Jun's heartbeat was a little fast, and he stared at the nude mouse in his hand without blinking.

He had never done a toxicity test for the alien drug until this moment. If it suddenly died, perhaps the experiment would have lost its meaning. And now, he stared at it for five minutes, followed by ten minutes...

Half an hour later, the nude mouse still showed no abnormalities.

"It seems that the Yiwen drug has no obvious acute toxicity." Gu Junsong breathed, "The chronic toxicity needs to be observed again."

Then he continued to fill the medicine with the next nude mouse. After completing the medicine with the other 7 nude mice in the three dosing groups for ten minutes, he observed for another hour. They all had no abnormal reactions, and they could still be kinetic, drink water and eat feed.

"Dear rat friends, let's live." Gu Jun muttered, pressing his painful head, his condition was obviously getting worse, "Please."

...

The next day and the third day, Gu Jun continued to work in the nude mouse room every day.

The tumors of the nude mice in Group B who were not given the drug had increased from 0.3cm to 0.6cm, and their weight and mental state had not changed. However, the tumor size of the two nude mice in Group A who were given the drug had not changed after two days and remained at 0.5cm.

Gu Jun knows what this may mean, and the varicose medicine is really effective! These capsules can significantly inhibit the growth of tumors.

Moreover, the other nude mice are administered as usual, which also means that the Yiwen medicine does not seem to have any adverse reactions and does not cause much damage to the body's functions.

"Maybe, I'm really saved this time?" He thought to himself, although the uneasiness still lingers in his heart, a new hope burned at the same time.

Day 4, day 5... A week passed in a blink of an eye.

The tumors of the nude mice in Group B have grown to 1.5 cm. The huge tumor forms a sharp contrast with its thin body. It begins to lose weight and become mentally unhealthy. The two nude mice in Group A continue to be in a stable condition, and all the nude mice given to the drug live well, which is no different from the nude mice in the blank control group.

I have to say that the results obtained in this experiment are so satisfying.

The efficacy of the heterologous drug is better than the top small-molecule targeted tumor drugs on the market.

However, Gu Jun also knew that the adverse reactions of Yiwen medicine cannot be completely ruled out, which only proved that it has no side effects in a short period of time. And this is an animal experiment after all, and there is a certain difference between it and human clinical experiments.

However, the results of this experiment are worth his risk.

That afternoon, after writing his suicide note, Gu Jun came to the waiting area of ​​the emergency department of Dongda Affiliated Hospital and sat down in a rare empty seat for waiting benches. The surroundings were crowded with patients and families, mostly crying children and comforting parents. The nurses hurriedly walked around, and the nurses pushed the rescue bed...

There was noisy, and the radio from the emergency department continued to call: "No. 182, Chen Ziyang. No. 183, Wang Yuxuan. No. 184, Huang Zixuan..."

After taking a breath, Gu Jun decided to throw a capsule of alien medicine into his mouth, took a sip of mineral water and swallowed it.
Chapter completed!