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Chapter 6 Ascites-type tumor-bearing mice

Cai Zixuan looked around and said, "The leader of our group is Wang Ruoxiang."

"That's the case?" Gu Jun frowned helplessly, thinking that it was something. "It's not surprising at all, okay, I'm surprised if it's not her."

"Yes, that's Wang Ruoxiang." Cai Zixuan sighed when he heard this.

Wang Ruoxiang is the class monitor of their class. She has strong abilities, beautiful people and good personality. Of course, the people who pursue her from the teaching building to the bureau building were from the teaching building. The rich man was one of them. She chased her so vigorously back then, but in the end, Wang Ruoxiang refused in public: "Sorry, we are not the same type of people, I won't like people like you."

Well, a tall, handsome, rich, and no matter how bad he is, he is still an eight-year-old man. He is actually "this kind of person"?

At that time, everyone said that thanks to the rich man Jun and the squad leader, they made them believe in love again.

"The squad leader is still single now." Cai Zixuan obviously didn't know how ugly she looked now.

"Don't look at me like this." Gu Jun laughed at himself, "It's all over." It's true that everything has passed. The rich man Jun likes the squad leader, and even more so that he wants to catch everyone's goddess. After all, it has nothing to do with those profound things.

"Is that right?" Cai Zixuan stopped talking about the squad leader. He looked at his watch, "Hey, it's almost 8 o'clock, I have to go to the laboratory."

"Let's go, I'm going to the experimental building too." Gu Jun said.

Cai Zixuan immediately showed a knowing look. He just said that he didn't care about the squad leader, so he turned around and wanted to join in the fun. It's so good to be young.

"What?" Gu Jun was really helpless. He wanted to explain but didn't know where to start, "Forget it..."

"Hao Jun, you need to say it, you understand. I understand it once was a waste of water."

"You understand a sponge."

The Medical Experiment Building is located in the northwest of the campus. It is a dozen white-walled brick building with no characteristics on the outside. Even opposite the door is a piece of disrepaired cement floor. However, there are laboratories with complete instruments and equipment in the building for scientific research by teachers and students.

"Here." After entering the building, Cai Zixuan walked in front and introduced the situation to Gu Jun as he walked.

The project title of the experiment conducted by their group is "Research on Photodynamic Treatment of Tumors with Several Photosensitizers". It is a study of tumor transplantation in mice, photodynamic treatment with different photosensitizers, and then observing tumor changes.

The funds allocated this time were only 30,000 yuan, which was a lot for an undergraduate research experiment, but a pure Photofrin photosensitizer imported cost tens of thousands of yuan. In order to make several more photosensitizers, Wang Ruoxiang made various calculations and saved funds to set up domestic imitation Photofrin group, Duotepofen group, ALA group, and a blank control group, a total of 5 groups of mice.

"The research plan and the project report were all handled by the team leader." Cai Zixuan couldn't help but sigh when he spoke, "It's really Wang Ruoxiang."

Although Professor Gu is a tutor, he only takes time to guide him occasionally. A big guy like him, it is his business to get high scores in SCI articles. Therefore, the Pioneer Cup still depends on the students themselves. The group leader does the most things, but it is an opportunity that everyone is eager to find.

Because if the paper is published in the experiment, Wang Ruoxiang is the first author, and Cai Zixuan and the others can only be ranked behind.

"We are still in the first stage, which is to establish an ascites-type tumor-bearing mouse model. It has been a week since we were incubated. Is it true today?"

Cai Zixuan made a lot of comments, and suddenly found that Gu Jun had not spoken for a long time, and seemed to frown when he heard the word "tumour"...

"Uh Haojun...you know, the success rate of modeling is not 100%.

Cai Zixuan scratched the bald area in front of his head. He really didn't know what he said just now. Gu Jun understood it.

"What can be 100% in the world?" Gu Jun lowered his head and covered his face. My friend, can I not learn from that amount yet?

"Indeed." Cai Zixuan was very touched by this sentence, and his face surged with vicissitudes, "All conditioned laws are like dreams and bubbles."

OK, Gu Jun can't understand now...

During the conversation, the two took the elevator to the eighth floor, and the laboratory used by their group was on this floor.

Walking along the corridor and outside the laboratory door, Cai Zixuan pushed open the door and walked in first. He saw that the team members had arrived, and he became the last one.

"Zixuan, I'm waiting for you."

“Huh?”

At this time, everyone saw a person who walked in, and the laboratory suddenly became quiet, leaving only the chirping of mice.

A rich man...?

Didn’t he say he dropped out of school and traveled around the world? Didn’t he say he died? Didn’t he say he…

"Hello everyone." Gu Jun waved his hands to everyone with a smile and greeted him, looking at Wang Ruoxiang, "Hello, squad leader."

Wang Ruoxiang stood by a laboratory bench. She had black medium and short hair and delicate melon-shaped face, although she was bare-faced, but she looked very beautiful. Her long figure and white coat were all heroic. When Wang Ruoxiang saw him, she frowned slightly, "Hello Gu Jun." Her voice was also very nice.

Gu Jun nodded and responded to her, "I've been away from the squad leader for three months, but he was no longer the same as before.

"Hao Jun had something to leave school before, but now he's back." Cai Zixuan explained to everyone and introduced to Gu Jun again: Xu Hai, a classmate in the clinical major, is also Zhang Haoran, a major in basic medicine, He Yuhan, a major in pharmacy, and a group of five people are there.

Cai Zixuan asked, "Squad leader, Hao Jun wants to take a look with us, isn't it a big deal?"

Wang Ruoxiang pursed her lips silently and said, "Just stand aside and don't make trouble."

The rich man. She muttered in her heart, remembering that at the beginning of school a few years ago, she had a superficial affection for him... Vomit. Such people are lazy and have all kinds of poisons. They cannot carve rotten wood, and cannot support mud on the wall... Mice are worse than mice! Maybe only if they are pulled to slice and study can they make contributions to medicine.

"No." Gu Jun stood aside obediently, but he always felt that Wang Ruoxiang had a bad look... he had an illusion.

There are already a row of mice raised cages on the experimental bench, and each cage has a group of white Kunming mice. This is the most commonly used experimental animal. It is very gentle and small. It weighs only 18-22g at 1-1.5 months and has a body length of less than 10cm, so it can be used for experiments.

Except for a cage of modeling mice, the rest are all blank mice, which Wang Ruoxiang just bought from the school's experimental animal center. Once the modeling is successful, they can come in handy.

The team members were all here, and everyone immediately started to do things.

Crack! Wang Ruoxiang put on rubber gloves and went to the modeling mouse cage to catch mice. These mice were marked with the numbering method. She caught the No. 1 mouse. Cai Zixuan and several others were responsible for assisting, recording, video and photography work.

"On the eighth day of fibrosarcoma in the S180 mice implanted in abdominal cavity." Wang Ruoxiang looked focused and serious. He gently pressed the obviously bulging abdomen of the No. 1 mouse on his hand. The mouse then let out a painful scream and struggled, but she held it firmly in her hand.

She said: "My No. 1 continues to grow ascites, and it is already a frog's belly, and it is very vibrant and does not need to be executed for the time being."

When the team members heard what she said, they were all excited and came over to see clearly, "Is this the 'frog belly'?" "Awesome."

"It's worthy of being a frog's belly, it's so big." Cai Zixuan sighed.

Although they are all eight-year-old, this is the first time to do such a real scientific research experiment.

"Oh." Gu Jun also came over to take a look, still understanding what they were doing.

The reason why ascites-type tumor-bearing mice was first created was to obtain a large number of highly active S180 tumor cells. It should be noted that the original cell line was very expensive, but one tube was only enough to inject one mouse, and its activity was very fragile. The four treatment groups they set up needed to cultivate hundreds of mice.

So it is to create first and then expand. Now that mice grow out of the frog's belly, they can "retract ascites".

"Gu Jun." Wang Ruoxiang suddenly turned her head and glanced at him, "Can you walk away? It's a hindrance."

"Yes." Gu Jun shrugged and walked away. Your aura was OK, and it could be hindered by one meter away from you.

Cai Zixuan and the others pretended that they didn't see it, and Wang Ruoxiang continued to operate the next step.

She stabbed the abdominal cavity of the No. 1 mouse with a 5ml syringe, and successfully pulled out a full tube of yellow clarified ascites, and then injected it into an Eppendorf cone-bottom centrifuge tube. She then took it to centrifuge with a centrifuge. Once centrifuged, she could immediately see a large amount of white precipitates appearing at the bottom of the centrifuge tube, which were tumor cells.

"The modeling of mouse No. 1 was successful." Wang Ruoxiang was relieved and happy, and smiled at the team members: "There are a large number of S180 tumor cells in ascites!"

"Yeah!" "Haha." They were all young people, no one paid attention to the feelings of the mice. They cheered, and Cai Zixuan praised: "Take the first step to success!"

The first step was successful, and then the cells were created in the treatment group of mice, and then photodynamic treatment was performed after they became tumors.

There are many things to do. They continue to work hard, remove the supernatant liquid from the centrifuge tube, resuspend the count, and adjust the cell concentration...

To maintain cell activity, all injections must be completed within one hour. So they are working on a modeling mouse to the next one.

"Adjust the cell concentration to 1×10^7/ml..."

"Yuhan, you are responsible for taking 0.2 ml of cells per needle."

"Team leader, the anesthetics are ready!"

Each of them was full of fighting spirit, and no one could care about the rich man and even forgot that he was standing aside and watching.

Gu Jun watched quietly, watching He Yuhan with cell suspension, Cai Zixuan anesthetized healthy mice with intraperitoneal injection of sodium pentobarbital, Xu Hai and Zhang Haoran marked the mouse and hair removal on their buttocks, and finally Wang Ruoxiang injected tumor cells subcutaneously into the right buttocks of the mouse...

No. 1, No. 2, No. 3, one after another.

Suddenly, Gu Jun's head slit and painful. Is it a brainstem tumor attack?

The pain suddenly intensified, and he couldn't help but scream in pain: "Ah!" The scene in front of him became dazed, flickering like the shadow of a nightmare.

He seemed to be unable to breathe the air, as if he was in a broken laboratory, with various instruments such as centrifuges, microscopes, sterilization pots, etc., and he fell to the ground. Broken test tubes, messy documents, blurred photo frames, rotten animal corpses... The dust turned all this into dark colors, without a breath of life, only chaos and silence.

The pain in the brain became more and more intense, but the scene became clearer. He saw some unspeakable devices clearly.

And the twisted corpses on the ground... they couldn't identify what animal they were...

He saw that there was a line of blood words on the wall inside, which was the mysterious language on the medicine box, but compared to the standard of printing, the lines of blood words had a terrifying weirdness.

In a trance, Gu Jun suddenly seemed to be able to understand the meaning of the blood-like word.
Chapter completed!
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