There are already several people busy in the germ laboratory.

Several rows of wooden shelves were filled with a wide range of glassware, and there were several microscopes on the long table, pools, operating tables, various knives and strange-shaped glassware, which looked very strange.

Although it was April, Ma Yilong couldn't help but shivered.

Pang Xian stepped forward and reported to Li Shizhen and Li Lunming who were studying in the microscope. Then they looked up at Zhao Hao.

"You are here just right, look at it." Li Shizhen greeted Zhao Hao excitedly.

"What did you find?" Zhao Hao walked over with interest, stood between Li Wan and the others, and pointed one eye at the microscope's eyepiece.

I saw a thin layer of solid culture medium in the glass petri dish... This is made of seafood cauliflower into transparent agar, and then a little nutritious gravy is added, which can not only cultivate bacterial growth, but also facilitate observation. This method has been used four hundred years later. In this time and space, it is naturally considered one of the many unremarkable scientific inventions of Mr. Zhao.

Of course, the culture medium is not the focus, the focus is on the small round dots on the culture medium.

"What kind of cocci is this?" Zhao Hao asked in a silence.

Li Lunming answered from the side: "We found this cocci from the suppurated wounds of different patients. According to the method taught by Gongzi, after dyeing with iodine, it was observed that it turned golden under the oil mirror. It should be what Gongzi called Staphylococcus aureus."

"Oh." Zhao Hao nodded. People who have a little common sense in later generations will know that this is the most common type of bacteria that can cause various types of infections, and it is also zoonotic. It is the biggest threat after a person is injured.

But for the doctors in the Ming Dynasty, this was an extraordinary discovery.

But obviously, this is not what makes Er Li excited, because this plate... Oh no, the cocci are not stained, so the purpose is naturally to avoid contamination test results!

"Young Master, look below." Seeing his eyes leaking, Er Li jumped up anxiously. "So obviously you can't see it?"

Under the guidance of the two, Zhao Hao discovered that there was a white circular area in the culture medium covered with cocci reproductive communities. Because it was too obvious, he ignored it.

"Is this the cocci melted?" Zhao Hao finally understood and immediately became interested: "How did you do it?"

There are too many things that can kill yeast cocci, such as alcohol and iodine, so it is not uncommon to do it. The key is how to do it.

"Use this!" Li Shizhen picked up a spotted orange.

"Damn, you separated the penicillin!" Zhao Hao was immediately excited. Oh my God, I finally waited for this day...

Why is Mr. Zhao always so cautious? He never drinks raw water on weekdays, and has to boil water before eating fruits and vegetables. He refuses to do intense activities and dares to do any dangerous actions. It is really because he is afraid of bacterial infection.

In this era of shortage of medical treatment and medicine, if you can't make a small hole, or eat bad things any time you eat, it will cause bacterial infection and die.

Last time he was broken at sea, Mr. Zhao was so worried that he almost cried...

So one of the main purposes of his establishment of Jiangnan Hospital... was to do everything possible to improve the medical level of the Ming Dynasty and increase his chance of survival when injuries hit.

Penicillin, a magical tool for traveling through life, is naturally the top priority in research and development.

He watched the Japanese drama "Ren Doctor" in his previous life, and still remembered the plot of southern doctors making penicillin through ancient times. At that time, he had Zhihu and said that there were three doctors in medicine who provided medical guidance for screenwriters, and that method should be reliable...

When Jiangnan Hospital was first established, Zhao Hao told three miraculous doctors about the method of extracting penicillin by doctors from southern China. Basically, they first sterilize the oranges and make them into culture medium. Then they inoculate the penicillium from the moldy oranges and continue to cultivate and reproduce.

After breeding to a certain amount, filter the culture medium with a funnel, add rapeseed oil to the filtered liquid, stir evenly in the barrel, and make the liquid layer separate - the upper oil-soluble substance, the intermediate insoluble substance, and the lower water-soluble substance.

Penicillin is a water-soluble substance. So by installing a faucet at the bottom of the barrel, you can get penicillin dissolved in water.

Next, pour it into activated carbon sterilized with boiling water and stir. Then filter it with distilled water to wash away the debris. Then filter it with acidic water mixed with white vinegar. Because penicillin is weakly acidic, it will not dissolve in acidic water, so alkaline impurities will be removed.

Finally, filter it with baking soda water, so that the result is a high-purity penicillin solution.

It sounds very simple, and Mr. Zhao also thinks it is not difficult. He thought it is a stupid method of "separation-choice-purification-choice". He can do miracles with great force. Oh no, there are many people and great power, so you can definitely extract penicillin.

But Jiangnan Hospital has been established for two years. The three miraculous doctors and their disciples worked day and night, and they have only made breakthroughs now...

But you don't have to worry about having a good meal.

"Hahaha!" In the laboratory, Mr. Zhao was laughing proudly: "I finally saw penicillin alive!"

~~

Of course, Ma Yilong didn't know why Mr. Zhao was so excited. He felt that as soon as he entered this room, he became an idiot.

Li Shizhen opened the curtains and shouted to the excited young master Zhao who was dancing with joy: "Calm down, don't break the equipment!"

"Uh..." Zhao Hao held a flask awkwardly and said with a dry smile: "Sorry, I'm so excited."

"Young Master, don't be too excited too early." Li Lunming smiled bitterly, "The Petri dish you saw is the only one that succeeded in this experiment."

"Where are the rest?" Zhao Hao asked hurriedly.

"It's all here." Li Lunming opened a cabinet and stacked hundreds of petri dishes neatly. "Before, we dripped the penicillin solution we obtained into these petri dishes, and only got a successful sample."

"This is the success of labor from the beginning of the year to the present," Li Shizhen said gloomyly: "It does prove that penicillin can be extracted from penicillin to kill bacteria, but after working for three months, the penicillin obtained can't be killed even in a petri dish. This method is a drop in the bucket for curing diseases."

"Spit is better than it." Li Lunming's temper had no choice but to spit into the Petri dish if he hadn't been wearing a mask.

But he was able to say such words, obviously he had tested the bactericidal effect of saliva...

"Don't do this, this is a good thing." Zhao Hao suppressed the disappointment in his heart and thought to himself that he had to continue to live... But as a cheerleader, he certainly had to smile and encourage the doctors and said, "Whatever he does is from nothing to something, from rough to essence? It's only been less than two years, and it's already from nothing to something. It's a joyful congratulation!"

"That's right." Li Shizhen, as the dean of the Medical School, had to maintain an optimistic attitude. "We did it for a year last year but it didn't come up with nothing. It was only in winter that progress began. Later, I found that the penicillin was not heat-resistant and did not breed at all in summer. Jiangnan was particularly long in summer..."

"Uh..." Mr. Zhao nodded secretly when he heard this, as if it was like this. But he seemed to have forgotten to say that in order to avoid being burned by Er Li's anger, he pretended to be in a daze and said, "No wonder I couldn't copy it back then. It turned out to be the reason."

"If the young master can discover penicillin, it's already amazing, okay?" Li Shizhen said with a look of dissatisfaction:

"There is a need for Penicillium of unknown origin to fall into your Staphylococcus culture medium. It was still cool early spring at that time, so Penicillium could grow and mature and produce penicillin. Then the temperature rose, which was conducive to the rapid growth of Staphylococcus, so that lytic disease occurred..." Li Lunming also looked at Zhao Hao with an incredible look and said, "And it had to be done for more than a month. If he was in medical school, he would have to be expelled eight times."

"This is called "no coincidence to make a book." Mr. Zhao said, "I'm afraid that Fleming found penicillin in such an extremely coincidental state."

However, due to the technical conditions at that time, Fleming was unable to isolate penicillin alone. It was not until before World War II that a little penicillin was isolated by the Americans. It is said that penicillin produced in the entire United States in one year was only available for one person to use...

Thinking about this, it was very normal for Jiangnan Hospital to work hard to produce this little penicillin.

"What are you going to do next?" Mr. Zhao asked without further ado.

"Stay trying, there are still many things that can be improved." Li Lunming said: "For example, purify the baking soda and see if it will be better."

"Well, this is a road." Zhao Hao used his brain to think for a moment: "But the key is to reproduce more penicillium. Perhaps citrus cultivation is not the best choice. It is not only citrus that have penicillium..."

"Uh..." Er Li looked at Zhao Hao speechlessly: "Why didn't you say it earlier?" They always thought that only Penicillium of citrus can do...

"I'm guessing too." Zhao Hao wiped his sweat and said with a sneer: "Didn't you say it earlier? You can't be superstitious about authority, you must be brave enough to try."

"Okay." The two nodded and silently considered where to extract the mold.

"Is this the progress you are asking me for?" Zhao Hao asked with some disappointment.

"No." Li Shizhen said lightly: "The allicin extraction has been successful."

"Damn it, don't you tell me earlier! I'll just suppress you if you want to show off..." Mr. Zhao immediately smiled.

Compared to the high-difficulty penicillin, it is much easier to extract allicin. Mash the garlic and let stand for one hour, then soak and extract it with high-concentration alcohol to obtain a low-concentration alcohol solution of allicin. Or it can be obtained by distilling water vapor. The allicin solution has a killing effect on a variety of cocci bacteria and also inhibits fungi. It is considered a low-end multi-purpose life-saving tool...

"Uh, sorry, interrupt..." Seeing that the three of them were about to discuss allicin again, Ma Yilong, who had been waiting for a long time, finally couldn't help asking: "Can this penicillin, and allicin, cure silkworm disease?"

"Uh..." The three people who were so hot that they remembered that there was one here.
Chapter completed!