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Chapter 98 Success

Many soldiers in Yizhou City are often fatal because of a small wound. During the war period when medical treatment is backward due to the raging disease, if they are unfortunately infected by bacteria, it is likely to mean death, even if they accidentally scratch a small wound at the beginning.

Some people say: The three major inventions in World War II - penicillin, mushroom bomb, and radar.

Penicillin is a type of antibiotic, which refers to a type of antibiotic that contains penicillane in the molecule, which can destroy the cell walls of bacteria and play a bactericidal role in the reproduction of bacterial cells. It is an antibiotic extracted from Penicillium. Mushroom eggs are powerful, and Japan's defeat depends on this. Not to mention radar, it uses radio methods to discover targets and determine their spatial location.

People have found high-efficiency bacterial species on moldy melons, and used technologies such as corn slurry fermentation to greatly increase the production of penicillin, and penicillin sold as a commercial drug was released.

But how could Zhu Cilang create these things? Melons are everywhere in this era, but corn has not yet been popularized.

Without corn, use other things instead. Because antibiotics are too important. In ancient times, when survival rates are low, antibiotics can not only change the battlefield pattern, but also save thousands of people's lives.

Sai Huatuo was very good at it. Although he didn't understand much theoretical knowledge, he understood it as soon as Zhu Cilang talked about the principle.

Chen Langzhong and several other military doctors have a group of elm-heads, and rotten wood cannot be carved. Although Zhu Cixuan explained to them countless times the principles of antibiotics and bacterial infections, they were still confused. They determined that it was evil poison and damaging poison.

No matter what, as long as they know that antibiotics can kill these wound-infected bacteria. How to create penicillin also requires experiments.

The production of penicillin is divided into two steps: strain fermentation and extraction and refining. It is not difficult to make strain fermentation. Because penicillin is widely distributed in nature.

Zhu Cixuan used sweet potato and rice soup as culture medium, inoculated Penicillium yeast-producing fungus onto solid culture medium, and cultured it in a room about 25 degrees for seven to ten days. At this time, a large amount of Penicillium spore culture can be obtained.

Then use distilled water to make the spores into a suspension and continue to culture. By stirring, the indoor temperature is slightly increased at the same time, and then the Penicillium culture medium is continued to add an appropriate amount of culture medium.

The difficulty is the extraction and refining of penicillin, and the penicillin fermentation liquid must be cooled and filtered.

First add rapeseed oil and stir. After stirring, extract the refined culture medium. Remove most of the incompatible and lipid-capacity substances.

Then, grind the charcoal into powder, add the refined culture medium to allow the charcoal to absorb penicillin. Place the charcoal that absorbs penicillin in a container such as a separation tube column, wash it with distilled water and acidic water, and then rinse it with alkaline water. Then the divided penicillin will be divided into a certain part, concentrated and then dissolved. This is the separation tube column chromatic layer separation method.

It seems simple, but it is actually extremely difficult. First of all, the success rate is very low. Only one or two bottles of penicillin can be extracted from ten bottles of solution.

Clean with distilled water, then inject acidic water made of vinegar and alkaline water made of grass ash. At this time, add filter cotton at the lower outlet to reduce the flow rate.

Zhu Cilang made it according to this method. However, there was a lot of waste of food. Unfortunately, most of the infected injured and sick people did not survive.

Zhu Cilang was a little annoyed. If he had made some penicillin in advance, the soldiers would not have suffered such great casualties.

"Xia Dechao, how is it?"

"Return to His Highness the Crown Prince, the lower official found two people infected with the poison in the city. Your Highness may try it on them."

Zhu Cilang is looking for bacterial infections, and there are two unlucky guys in the city. One is a pig killer, but a small wound was cut by a pig killer knife, causing secondary infection to be unable to see it.

Another one was also scratched by an iron tool, and both of them were secondary to bacterial infection. The doctor had used many Chinese medicines, but they were useless.

When Zhu Cixuan brought two bottles of penicillin, the two patients had been sentenced to death. Even their families had prepared for their funeral.

Several military doctors were arrested by Zhu Cilang. This magical little prince opened up a new medical world that was unprecedented in history for them. Especially Sai Hua Tuo, he worshipped Zhu Cilang like a god.

To be honest, Zhu Cilang didn’t know if it worked. Very few of them were the dose of a bottle of penicillin the size of a thumb.

This thing needs to be done for skin test, but Zhu Cixuan did not do it. Anyway, the dead horse is treated as a living horse, and these two people are dead without this medicine, so the steps for skin test are directly ignored.

When getting the injection, these military doctors were also eye-opening. In fact, Zhu Cilang also felt the stones across the river, and shot the buttocks of the two wounded.

"Your Highness, is this true?" Sai Huatuo asked in surprise.

Zhu Cilang was not sure. He thrust his waist and looked at the two half-dead patients: "Yes, it should be done."

What does it mean? These two half-dead guys, can this cure after giving them these two injections?

I have never heard of decoction and external application to treat injuries, and I have never heard of what to do to give people injections and spanking.

These two patients who entered the body of evil poison were helpless. Maybe the little prince would be unexpected and heal them.

"What, when will it be effective? Your Highness, this person." Chen Langzhong also asked curiously.

"Tomorrow, if the condition improves, you can see it tomorrow." Zhu Cilang replied without any confidence.

The next day, unfortunate news came. Zhu Cixuan gave two patients who had two injections, one of whom died that night. The family pulled the body back and cried all the way back to bury him.

When the news of luck came, another pig slayer miraculously improved. Logically, his condition was worse than the other one, but he became alive.

This news made Zhu Cilang very excited. In other words, one of the two bottles of penicillin was effective.

Sai Huatuo even forgot to pay his respects, and ran into Zhu Cixuan's room disrespectfully, then he remembered his rudeness: "The villain deserves to die, the villain is rude. His Royal Highness, the prince is alive, and one of the patients is alive."

In order to wait for news about these two patients, Zhu Cixuan did not sleep well all night. When he heard Sai Hua Tuo shouting, he hurriedly stood up and said, "Let's go, take me to see."
Chapter completed!
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